• Published
    25-02-2024
  • Issue
    Vol: 28 Issue: 01, 2024
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ANTIMICROBIAL ACTIVITY OF METHANOL EXTRACT OF CAESALPINIA SAPPAN AGAINST PSEUDOMONAS AERUGINOSA

  • Vikraman K S, Dr. P. Sankar Ganesh, Dr. A.S. Smiline Girija, Dr. J. Vijayashree Priyadharshini

Keywords: Caesalpinia sappan, Pseudomonas aeruginosa, MHA agar, Environment.

ABSTRACT:-
Background: According to the World Health Organization (WHO) more than 80% of the world's population relies on traditional medicine for their primary healthcare needs. Use of herbal medicines in Asia represents an extended history of human interactions with the environment. Caesalpinia sappan Linn. a little thorny tree, 6-9 m high is found in India, is a well-established alternative medicine to treat various ailments. Pseudomonas infections are diseases caused by a gram-negative bacterium from the Pseudomonas and is considered as a potent nosocomial pathogen with multi-drug resistant properties. Methods: C.sappan (10g) was collected and mixed with 100 ml of methanol and kept in a shaker and the extract was filtered using Whatman No:1 filter paper and crude extract was used for further study. MHA agar plate was used and C.sappan was loaded in each well of different concentrations. After 24 hours of incubation the zone of inhibition was measured using a standard scale. Results: The result showed that 10 μL, 20 μL, 30 μL and 40 μL concentrations of methanol extract of Caesalpinia sappan has predominantly inhibited Pseudomonas aeruginosa in a concentration dependent manner. Conclusion: The present study showed that low concentration of methanol extract of C.sappan inhibited the growth of Pseudomonas aeruginosa. The methanol extract of C. sappan is of potential value as natural antimicrobial inhibitors and could be used safely for treatment of Gram-negative bacterial infections and also can be used safely in the food industry.